Volume 6, Issue 1, February 2020, Page: 26-32
Characterization of Advanced Hexaploid Wheat Lines Against Stripe Rust (Puccinia striiformis f. Sp. tritici) and Identification of Employed Pathogen Races
Getnet Muche, Kulumsa Agricultural Research Center, Ethiopian Institute of Agricultural Research, Asella, Ethiopia
Alemu Ayele, Kulumsa Agricultural Research Center, Ethiopian Institute of Agricultural Research, Asella, Ethiopia
Received: Dec. 18, 2019;       Accepted: Jan. 12, 2020;       Published: Feb. 12, 2020
DOI: 10.11648/j.ajme.20200601.14      View  570      Downloads  136
Wheat is one of the world's most important crops whose grain production is increasing year after year. However, its production is badly constrained by wheat rusts. Stripe rust caused by Puccinia striiformis f. sp. tritici is an important disease of wheat resulting significant yield failure in wheat growing areas around the globe. The pathogen is one of the very important yield limiting factors in Ethiopia. The severity is worse due to emergency of virulent stripe rust races at one point of the world spread to the rest of wheat producing countries by wind and human travels. Thus, development and cultivation of hereditarily diverse and tolerant varieties is the most sustainable option to overcome these diseases. The present study was carried out with the aim to identify possible sources of stripe rust resistance among Ethiopian bread wheat breeding pipelines to enhance cultivar improvement efforts and identify physiologic races involved during screening process. A total of four mono-pustule isolates were collected from Meraro and Kulumsa, stripe rust hot spot locations. Out of these, two P. striformis races; namely, PstS2 and PstS11 were identified. The former was detected at Meraro and virulent to seven of the 19-diffential lines while PstS11 displayed across Meraro and Kulumsa and virulent to nine of the19-diffential lines. Twenty-eight advanced bread wheat pipelines and a universal susceptible cultivar, Morocco were evaluated for their resistance at the seedling stage against identified stripe rust races (PstS2 and PstS11) in a controlled environment. Of the 28, twenty and seventeen lines exhibited susceptible seedling reactions to PstS2 and PstS11 with infection types ranging from 7 to 9, respectively. Those groups of lines that showed susceptible reaction at seedling stage are expected to possess poly minor genes that could be used for durable stripe rust resistance breeding in wheat. However, is advised to evaluate for adult plant resistance and postulate inherent resistance genes in these lines for fruitful recommendations.
Inherent Genes, Pathogen Races, Seedling Resistance, Slow Rusting, Yellow Rust
To cite this article
Getnet Muche, Alemu Ayele, Characterization of Advanced Hexaploid Wheat Lines Against Stripe Rust (Puccinia striiformis f. Sp. tritici) and Identification of Employed Pathogen Races, American Journal of Modern Energy. Vol. 6, No. 1, 2020, pp. 26-32. doi: 10.11648/j.ajme.20200601.14
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
INRA (International Research Associates) “Wheat initiative: An international vision for wheat improvement,” 2013. [Online]. Available: www.wheatinitiative.org, [Accessed 27 May 2014].
FAO (Food and Agriculture Organization) (2015a). Food Balance Sheets. FAOSTAT. Rome, Italy.
USDA. 2019. United States Department of Agriculture. World Agricultural Production. Circular series report. May 2018.
CSA. 2019. Agricultural sample survey: Report on area and production of major crops, Central Statistical Agency, Addis Ababa, Ethiopia, 18 Pp.
Hailu, G., Tanner, D. &Mengistu, H., 2011. Wheat research in Ethiopia: A Historical perspective. IARI and CIMMYT, Addis Ababa.
Tesfaye K. and Pim E., 2016. CIMMYT gathers partners to discuss biotic stress and cropmodel integration [Online]. [Accessed 30 October 2018].
Boyd, LA (2005) Centenary review: Can Robigus defeat an old enemy? – Yellow rust of wheat. J Agric Sci 143: 1–11.
Fetch T., McCallum B. Menzies J., Rashid K. and Tenuta A. 2011. Rust diseases in Canada. Prairie Soils and Crops Journal. 4: 86-96.
Beddow J. Pardey G. Chai Y., Hurle M., Kriticos J. and Braun J. C. 2015. Research investment implications of shifts in the global geography of wheat stripe rust. Nat. Plants1: 15132. 10.1038/nplants.2015.132.
Worku D., Bekele H., GetanehW., Endale H., Bekele K., Teklay A., Daniel K., Tilahun B., EbabuyeY, Mekonen A., Ashenafi G., Seyoum Z., Nigussie D., BitewB., Handor F. and Girma K. 2014. Monitoring wheat stripe rust in Ethiopia: The 2011 and 2012 trap nurseries and stripe rust virulence surveys. 2nd international wheat stripe rust.
Ayele B., Solomon A. Fehrmann H. 2008a. Yellow rust resistance in advanced lines and commercial cultivars of bread wheat from Ethiopia. East African Journal of Science. 2: 29-34.
Wubit D., Flath K. Weber E., Schumann E. Röder M. S. and Chen X. 2012. Postulation and mapping of seedling stripe rust resistance genes in Ethiopian bread wheat cultivars. Journal of Plant Pathology. 94: 2, 403-409.
Teklay A. 2014. Variability in stripe rust occurrence in Tigray, Ethiopia. 2nd international wheat stripe rust symposium: regional cereal rust research center Izmir, Turkey. PP; 7.
Eshetu B. 1986. Review of research on diseases of barley, tef and wheat in Ethiopia. In: Tsedeke Abate (ed). A review of crop protection research in Ethiopia. Proceedings of the first crop protection symposium. IAR, Addis Ababa Ethiopia. Pp 79-108.
Atilaw A., ZelalemB., Finisa E., Solomon G., ZerihunT., Aliye S., Abdalla O., Asnake F., Ahmed S. and Silim S. 2014. Controlling wheat rusts and ensuring food security through deployment of resistant varieties in Ethiopia. 2nd international wheat stripe rust symposium regional cereal rust research center Izmir, Turkey. 14 Pp.
Chen XM (2007) Challenges and solutions for stripe rust control in the United States. Aust J Agric Res 58: 648–655.
Bekele H. 2003. Short report on stripe rust and stem rust. In: BedadaGirma. (eds.). Proceedings of the agronomy workshop, Bale Agricultural Development Enterprise (BADE). Addis Ababa, 67-78.
Line RF, Qayoum A (1992) Virulence, aggressiveness, evolution, and distribution of races of Pucciniastriiformis (the cause of stripe rust of wheat) in North America, 1968- 87. US Dep. Agric. Agric. Res. Serv. Tech. Bull. 1788.
ICARDA. 2011. Strategies to reduce the emerging wheat stripe rust disease: Synthesis of a dialog between policy makers and scientists from 31 countries at; International Wheat Stripe Rust Symposium, Aleppo, Syria, April 2011. PP 1-23.
Ayele B. 2002. Breeding bread wheat with multiple disease resistance and high yield for the Ethiopian highlands: Broadening the genetic basis of yellow rust and tan spot resistance. Ph. D Thesis. Cuvillier Verlag Goettingen, Germany, 115pp.
Birhan A. 2011. KARC stations distribution and website description (Un published data).
Stubbs RW., Prescott JM., Sarrri EE. And Dubin HJ. 1986. Cereal Disease Methodology Manual. El Batan, Mexico, CIMMYT.
Fetch T. G. and Dunsmore K. M. 2004. Physiological specialization of P. graminison wheat, barley and oat in Canada. Canadian Journal of Plant Pathology. 26: 148-55.
Johnson R., Stubbs RW. Fuchs E. and Chamberlain NH. 1972. Nomenclature for physiological races of Puccinia striiformis infecting wheat. Trans Sr Mycol Soc. 58: 475–480.
McNeal F., Konzak H., Smith E. P., Tale W. S. and Russel T. S. 1971. A Uniform System for recording and processing cereal research data. U. S. Department of Agricultural service. Article, Pp, 34-121.
Ali S. Muhammad R., Gautier A., Zahoor A. and Walter S. 2017. Microsatellite Genotyping of the Wheat Yellow Rust Pathogen Pucciniastriiformis. Wheat Rust Diseases: Methods and Protocols in Molecular Biology, vol. 1659, DOI 10.1007/978-1-4939-7249-4.
Hovmøller M. S., Rodriguez-Algaba J., Thach T. and Sørensen C. K. 2017. Race Typing of P. striiformison Wheat. In: Sambasivam Periyannan (ed.), Wheat Rust Diseases.
Justesen A. F., Ridoutb C. J., and Hovmoller M. S. 2002. The recent history of Pucciniastriiformisf. sp. tritici in Denmark as revealed by diseases incidence and AFLP markers. Plant Pathol. 51: 13-23.
Ali S., Gautier A., Leconte M., Enjalbert J. and de Vallavieille-Pope C. 2011. A rapid genotyping method for an obligate fungal pathogen, Pucciniastriiformisf. sp. tritici, based on DNA extraction from infected leaf and Multiplex PCR genotyping.
Rodriguez-Algaba J., Walter S., Sørensen CK., Hovmøller MS., Justesen AF. 2014 Sexual structures and recombination of the wheat rust fungus Pucciniastriiformis on Berberis vulgaris. Fungal Genet Biol. 70: 77–85.
Hovmøller M. S., Rodriguez-Algaba J., Thach T. and Sørensen C. K. 2016. Race typing of P. striiformison Wheat. In: Sambasivam Periyannan (ed.), Wheat Rust Diseases: Methods and Protocols, Methods in Molecular Biology. 1659: 29-40.
Hovmøller M., Rodriguez-Algaba J., Thach T., Justesen A. F. and Hansen G. H. 2018. Report for Pucciniastriiformisrace analyses and molecular genotyping 2018. Global Rust Reference Center (GRRC), Aarhus University, Flakkebjerg, DK-4200 Slagelse, Denmark 10 February, 2018.
Bekele H., Netsanet B., Ayele B., Getaneh W., Dave H., Yoseph A., Bedada G. and Bekele A. 2018. Overview of Wheat Rust Epidemiology and Management in Ethiopia. Proceedings of the 24thannual conference for plant protection society of Ethiopia. March 16-17, 2018; Haramaya, Ethiopia, pp. 83-116.
Walter S., Ali S., Kemen E., Nazari K., Bahri BA., Enjalbert J., Hansen JG., Brown JKM., Sicheritz Ponte´n T., Jones J., de Vallavieille-Pope C., Hovmøller MS. and Justesen AF. 2016. Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis. Ecol. Evol. 9: 2790–2804.
Singh RP., William HM., Huerta-Espino J. and Rosewame G. 2004a. Wheat rust in Asia: meeting the challenges with old and new technologies. New directions for a diverse planet. In: Fischer T., Turner N., Angus J., McIntyre L., Robertson M., Borrell A., and Lloyd D. (eds); Proceeding of 4th International Crop Science Congress. Brisbane, Australia, 26 September–1 October 2004. Brisbane, Australia, pp 1–13.
Wan AM. and Chen XM. 2012. Virulence, frequency, and distribution of races of Pucciniastriiformis f. sp. triticiand Pucciniastriiformisf. sp. hordeiidentified in the United States in 2008 and 2009. Plant Disease. 96: 67-74.
Yahyaoui A. H., Hakim M. S., Naimi M. El. and Rbeiz N. 2002. Evolution of physiologic races and virulence of Puccinia striiformis on wheat in Syria and Lebanon. Plant Disease. 86: 499-504.
Chen X. M. 2005. Epidemiology and control of stripe rust (Puccinia striiformis f. sp. tritici) on wheat. Canadian Journal of Plant Pathol. 27: 314-337.
Singh RP, Huerta-Espino J. and William HM. 2005. Genetics and breeding for durable resistance to leaf and stripe rusts in wheat. Turk. J. Agric. 29: 121–127.
Dehghani H. and Moghaddam M. 2004. Genetic analysis of latent period of stripe rust in wheat seedlings. Journal of Phytopatholo. 122: 325-330.
Browse journals by subject